Kurdyukov Evgeniy Evgen'evich, Senior lecturer, sub-department of general and clinical pharmacology, Medical Institute, Penza State University (40 Krasnaya street, Penza, Russia), E-mail: email@example.com
Background. Diabetes mellitus is the most acute medical and social problem in almost all countries of the world. It is difficult to overestimate the importance of finding reasonable approaches to the prevention of diabetes. Stevia leaves, leaf extracts are used for the prevention of diabetes, and in the treatment of the disease. However, pharmacognostic aspects are insufficiently studied. In this regard, it is important to conduct phytochemical analysis of plant raw materials of stevia. Objective: to prove the presence and quantify the content of diterpene glycosides in stevia leaves.
Materials and methods. The material for use was samples of crushed raw materials from dried leaves of Ramon slasten variety grown in the conditions of the Penza region, Tver region, Krasnodar region, the Republic of Crimea. Identification of diterpene glycosides in stevia raw materials was performed by thin-layer chromatography (TLC). Quantitative determination of the amount of diterpene glycosides in extracts from stevia leaves was carried out by direct spectrophotometry.
Results. For the qualitative determination of stevioside, the TLC technique was applied. The detection was carried out visually in daylight after treatment of plates with 50% sulfuric acid solution. The spots were painted black. Typical area was the spot with Rf of 0.39, corresponding to stevioside. Direct spectrophotometry was used for screening analysis of the content of glycosides complex in stevia leaves. Stevia syrup with a known stevioside content was used as a comparison solution, the absorption linearity at 203±2 nm from the concentration was proved. For extracts from stevia leaves, the presence of a characteristic maximum at a wavelength of 203±2 nm is noted.
Conclusion. The maximum self - absorption of diterpene glycosides of alcohol extracts from stevia leaves-203 nm (maximum) was determined. It was found that the content of the sum of diterpene glycosides in stevia leaves varies from 14.8 to 21.4 %.
1. Lemus-Mondaca R., Vega-Gálvez A., Zura-Bravo L., Kong Ah-Hen. Food Chemistry. 2012, vol. 132 (3), pp. 1121–1132.
2. Madan Swati, Ahmad Sayeed, Singh G N., Kohli Kanchan, Yatendra Kumar Raman Singh and Garg Madhukar Indian Journal of Natural Products and Resources. 2010, vol. 1 (3), pp. 267–286.
3. Kurdyukov E. E., Semenova E. F. Nauchnye vedomosti Belgorodskogo gosudarstvenno-go universiteta. Ser.: Meditsina i farmatsiya [Scientific bulletin of Belgorod State University. Series: Medicine and pharmacy]. 2017, no. 26, pp. 137–145.
4. Fedorov S. V., Kedik S. A., Lnul' N. A. Biologicheski aktivnye dobavki k pishche i problemy optimizatsii pitaniya: materialy VI Mezhdunar. simpoziuma [Bioactive food supplements and problems of diet optimization: proceedings of VI International symposium]. Sochi, 2002, p. 266.
5. Kedik S. A., Yanul' N. A., Fedorov S. V. Pitanie i zdorov'e. Biologicheski aktivnye dobavki [Nutrition and health. Bioactive food supplements]. 2002, no. 8, pp. 42–44.
6. Kedik C. A., Fedorov S. V., Yanul' N. A., Prokhorova L. V., Smirnova E. V., Panov A. V. Khimiko-farmatsevticheskiy zhurnal [Chemical and pharmaceutical journal]. 2003, vol. 37, no. 10, pp. 19–22.
7. Rudakov O. B., Verzilina N. D., Fedorov S. V., Polyanskiy K. K. Molochnaya promyshlennost' [Milk industry]. 2004, no. 7, pp. 54–55.